Kopeikina A.A., Maltsev M.V. MODIFIED METHOD FOR DETECTION OF ANTIBIOTICS IN ANIMAL PRODUCTS BY LIQUID CHROMATOGRAPHY
DOI: https://doi.org/10.15688/nsr.jvolsu.2025.3.5
UDC: 574.2(470.45)
Alexandra A. Kopeikina, Master’s Student, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation, This email address is being protected from spambots. You need JavaScript enabled to view it.
Mikhail V. Maltsev, Candidate of Sciences (Biology), Associate Professor, Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation, This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract. The study aimed to improve methods for monitoring residual amounts of tetracycline antibiotics in food products, namely poultry meat. The relevance of this work is due to the need to ensure consumer safety and compliance with regulatory requirements for the content of antibiotics in food products. To achieve these goals, a new analysis technique was developed that includes modification of chromatographic separation conditions. In particular, experiments were conducted to optimize the parameters of high-performance liquid chromatography (HPLC), such as the choice of mobile phase, gradient change in solvent composition, column temperature and flow rate. These changes significantly improved the separation of components, increased sensitivity and selectivity of the method. Particular attention was paid to the use of tandem mass spectrometry (MS/MS), which provides high accuracy of identification and quantitative determination of antibiotics even at very low concentrations. As a result, conditions were obtained that allow simultaneous determination of several types of tetracycline antibiotics with high accuracy and reproducibility. The tests showed that the new method has a number of advantages: it provides a lower detection limit, increased selectivity due to improved separation of components, and reduces the analysis time. This makes it more effective for practical use in laboratory conditions. To confirm the effectiveness of the new method, comparative studies were performed on real samples of food products. The results showed a good correlation. In general, the presented development demonstrates the possibility of increasing the accuracy and reliability of monitoring the residues of tetracycline antibiotics in food products due to the use of improved HPLC-MS/MS conditions. The introduction of this method into practice will improve the level of food safety, promptly identify excesses of permissible standards and ensure the protection of consumer health on.
Key words: antibiotics, liquid chromatography, agriculture, animal husbandry, tetracycline group.
Citation. Kopeikina A.A., Maltsev M.V. Modified Method for Detection of Antibiotics in Animal Products by Liquid Chromatography. Prirodnye sistemy i resursy [Natural Systems and Resources], 2025, vol. 15, no. 3, pp. 43-49. (in Russian). DOI: https://doi.org/10.15688/nsr.jvolsu.2025.3.5
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