Pavel A. Krylov, Aliya A. Abdulova, Oleg V. Permyakov, Valery V. Novochadov STRUCTURAL AND FUNCTIONAL ANALYSIS OF MANGANESE- AND ZINC-DEPENDENT PROTEINS IN PLANTS GROWING IN THE SOUTH OF RUSSIA

DOI: https://doi.org/10.15688/nsr.jvolsu.2022.1.6  

Krylov Pavel Andreevich, Candidate of Sciences (Biology), Associate Professor, Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation, This email address is being protected from spambots. You need JavaScript enabled to view it.
Abdulova Aliya Alikovna, Student of Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation. This email address is being protected from spambots. You need JavaScript enabled to view it.
Permyakov Oleg Valer’evich, graduate student of Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation.  This email address is being protected from spambots. You need JavaScript enabled to view it.  
Novochadov Valery Valer’evich, Doctor of Sciences (Medicine), Professor, Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian
Federation, This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract. Modern bioinformatic approaches allow structural and functional analysis of proteins of various organisms for the presence of homologous and non-homologous sites. This study is aimed at identifying structural and functional differences of manganese- and zinc-dependent proteins in common plant species growing on the territory of arid biocenoses of Southern Russia. The main purpose of the work was to study the presence of manganese- and zinc-dependent proteins included in the proteome of common plant species typical of arid territories of Southern Russia. As a result of the analysis of the plant proteome for the presence of manganese- and zinc-dependent proteins, it was found that the degree of study was very low, the number of annotated proteins was 5 pieces only for Artemisia annua and Artemisia lercheana, while there was no protein annotation at all for other species. Later, using a bioinformatic search, the presence of a manganese-permeable Photosystem II protein D1 protein and a zinc-dependent Acetyl-CoA carboxylase carboxyl transferase subunit beta was found in all plants. Structural analysis of sequences using multiple alignment showed that there are strong structural differences in the manganese-dependent Photosystem II protein D1 protein between Artemisia lercheana, Artemisia annua, Cichorium intybus, Descurainia Sophia, Tripleurospermum inodorum and Xanthium albinum, and for the zinc-dependent protein Acetyl-CoA carboxylase carboxyl transferase subunit beta in Artemisia lercheana, Artemisia annua, Cichorium intybus and Descurainia sophia, which is associated with a variety of amino acid substitutions, including conservative and weakly conservative sites. The obtained results of the study showed a low degree of knowledge and description of the structural and functional properties of metal-dependen.

Key words: metal-dependent proteins, manganese, zinc, bioinformatics, multiple alignment, plants of arid biocenoses.

Citation. Krylov P.A., Abdulova A.A., Permyakov O.V., Novochadov V.V. Structural and Functional Analysis of Manganese- and Zinc-Dependent Proteins in Plants Growing in the South of Russia. Prirodnye sistemy i resursy [Natural Systems and Resources], 2022, vol. 12, no. 1, pp. 45-53. DOI: https://doi.org/10.15688/nsr.jvolsu.2022.1.6

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