Pavel A. Krylov, Aliya A. Abdulova, Oleg V. Permyakov, Valery V. Novochadov STRUCTURAL AND FUNCTIONAL ANALYSIS OF MANGANESE- AND ZINC-DEPENDENT PROTEINS IN PLANTS GROWING IN THE SOUTH OF RUSSIA
DOI: https://doi.org/10.15688/nsr.jvolsu.2022.1.6
Krylov Pavel Andreevich, Candidate of Sciences (Biology), Associate Professor, Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation,
krylov.pavel@volsu.ru
Abdulova Aliya Alikovna, Student of Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation.
iananash@mail.ru
Permyakov Oleg Valer’evich, graduate student of Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian Federation.
bot@volsu.ru
Novochadov Valery Valer’evich, Doctor of Sciences (Medicine), Professor, Department of Biology and Bioengineering, Volgograd State University, Prosp. Universitetsky, 100, 400062 Volgograd, Russian
Federation,
novochadov.valeriy@volsu.ru
Abstract. Modern bioinformatic approaches allow structural and functional analysis of proteins of various organisms for the presence of homologous and non-homologous sites. This study is aimed at identifying structural and functional differences of manganese- and zinc-dependent proteins in common plant species growing on the territory of arid biocenoses of Southern Russia. The main purpose of the work was to study the presence of manganese- and zinc-dependent proteins included in the proteome of common plant species typical of arid territories of Southern Russia. As a result of the analysis of the plant proteome for the presence of manganese- and zinc-dependent proteins, it was found that the degree of study was very low, the number of annotated proteins was 5 pieces only for Artemisia annua and Artemisia lercheana, while there was no protein annotation at all for other species. Later, using a bioinformatic search, the presence of a manganese-permeable Photosystem II protein D1 protein and a zinc-dependent Acetyl-CoA carboxylase carboxyl transferase subunit beta was found in all plants. Structural analysis of sequences using multiple alignment showed that there are strong structural differences in the manganese-dependent Photosystem II protein D1 protein between Artemisia lercheana, Artemisia annua, Cichorium intybus, Descurainia Sophia, Tripleurospermum inodorum and Xanthium albinum, and for the zinc-dependent protein Acetyl-CoA carboxylase carboxyl transferase subunit beta in Artemisia lercheana, Artemisia annua, Cichorium intybus and Descurainia sophia, which is associated with a variety of amino acid substitutions, including conservative and weakly conservative sites. The obtained results of the study showed a low degree of knowledge and description of the structural and functional properties of metal-dependen.
Key words: metal-dependent proteins, manganese, zinc, bioinformatics, multiple alignment, plants of arid biocenoses.
Citation. Krylov P.A., Abdulova A.A., Permyakov O.V., Novochadov V.V. Structural and Functional Analysis of Manganese- and Zinc-Dependent Proteins in Plants Growing in the South of Russia. Prirodnye sistemy i resursy [Natural Systems and Resources], 2022, vol. 12, no. 1, pp. 45-53. DOI: https://doi.org/10.15688/nsr.jvolsu.2022.1.6
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