Candidate of Biology, Assistant, Department of Bioengineering and

Bioinformatics, Volgograd State University

Prosp. Universitetskij, 100, 400062 Volgograd, Russian Federation

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Student, Department of Bioengineering and Bioinformatics, Volgograd    State University

Prosp. Universitetskij, 100, 400062 Volgograd, Russian Federation

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Student, Department of Bioengineering and Bioinformatics, Volgograd State University

Prosp. Universitetskij, 100, 400062 Volgograd, Russian Federation

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Student, Department of Bioengineering and Bioinformatics, Volgograd State University 

Prosp. Universitetskij, 100, 400062 Volgograd, Russian Federation

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Doctor of Science (biology), Associate Professor, Head of Department

of Bioengineering and Bioinformatics, Volgograd State University

Prosp. Universitetskij, 100, 400062 Volgograd, Russian Federation

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Doctor of Science (medicine) Professor, Director of the Institute of Natural Sciences, Volgograd State University 

Prosp. Universitetskij, 100, 400062 Volgograd, Russian Federation

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Abstract. The use of modern bioinformatic approaches for the solution of environmentally oriented tasks provides new data that can be used for spot control anthropogenic ecosystems and ecosystem rebuilding of their violation obtained from adverse factors of natural and antropogenical genesis. This work aims at demonstrating and identifying nickel and cobalt binding proteins in dominant species of microorganisms of agrocenosis that allows you to create an information database for accumulating and processing information about the regularities of the functioning of agro-ecosystem in arid conditions, leading to the growth of economic efficiency of the farming system and increase the success of environmental management. The main purpose of the work was to study the presence of nickel and cobalt binding proteins included in the proteome, the dominant species of microorganisms from three typical agrocenoses of the Volgograd region. Nickel and cobalt binding proteins were distributed not only in their functional features, participation in metabolism, proteolytic activity, transport and regulation of gene expression and proteins, The results of virtual screening proteome the dominant microorganisms of the genus Bacillus and Actinomyces in the Uniprot database showed that the soil microbiota of the considered agrocenosis is also characterized by a sufficiently large number of metal – dependent proteins: 264 – for representatives of the Genus Actinomyces and 564 – for Bacillus subtilis, of of which about 35–40 % are annotated. According to the results, the concentration of metals in the soil of agrocenosis, namely in the arid zone, has a very strong impact on the livelihoods of crops and microorganisms in the soil. The use of the obtained results can be used as a basis for the implementation of targeted high-precision management of biocenoses in order to improve the sustainability of natural communities and the economic efficiency of agrocenoses.

Key words: agrocenoses, proteome, nickel, cobalt, metal binding proteins, agricultural nature management, database.