Grigoryeva D.V., Gorudko I.V., Lutsenko V.E., Cherenkevich S.N., Panasenko O.M., Sokolov A.V. Evaluation of the Functional Condition of Human Blood Neutrophills Based on the Registration of Active Halogen Forms Production

DOI: https://doi.org/10.15688/jvolsu11.2018.1.8

 
Darya V. Grigoryeva 
Senior Researcher, Department of Biophysics, Research Laboratory of Biophysics and Biotechnology, Belarus State University
Prosp. Nezavisimosti, 4, 220030 Minsk, Republic of Belarus
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Irina V. Gorudko
Leading Researcher, Department of Biophysics, Research Laboratory of Biophysics and Biotechnology, Belarus State University
Prosp. Nezavisimosti, 4, 220030 Minsk, Republic of Belarus
 
Veronika E. Lutsenko
Department of Biophysics, Research Laboratory of Biophysics and Biotechnology, Belarus State University
Prosp. Nezavisimosti, 4, 220030 Minsk, Republic of Belarus
 
Sergey N. Cherenkevich
Doctor of Sciences (Biology), Professor, Department of Biophysics, Research Laboratory of Biophysics and Biotechnology, Belarus State University
Prosp. Nezavisimosti, 4, 220030 Minsk, Republic of Belarus
 
Oleg M. Panasenko
Doctor of Sciences (Biology), Head of Department of Biophysics, Federal Scientific and Clinical Center for Physical and Chemical Medicine
Malaya Pirogovskaya St., 1а, 119435 Moscow, Russian Federation
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Aleksey V. Sokolov
Senior Lecturer, Faculty of Dentistry and Medical Technology, Saint Petersburg
State University
Universitetskaya Emb., 7-9, 199034 Saint Petersburg, Russian Federation
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Abstract. The development of adequate methods for monitoring the functional state of neutrophils is an 
urgent task due to their important role in the development of the inflammatory response. In this study, the functional 
activity of neutrophils has been assessed using a complex approach, including the registration of generation of 
ROS by neutrophils as a result of the functioning of the NADPH oxidase complex; degranulation of azurophilic 
granules; as well as production of AFG, formed in reactions catalyzed by the enzyme azurophilic granules of 
neutrophils – MPO in the presence of substrates (Н2О2 and halide ions).
 
Key words: neutrophils of human blood, production of active forms of halogens, functional activity of neutrophils.
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Evaluation of the Functional Condition of Human Blood Neutrophills Based on the Registration of Active Halogen Forms Production by Grigoryeva D.V., Gorudko I.V., Lutsenko V.E., Cherenkevich S.N., Panasenko O.M., Sokolov A.V. is licensed under a Creative Commons Attribution 4.0 International License.
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