Verle O.V., Ostrovskiy O.V., Verovskiy V.E., Dudchenko G.P. Estimation of the Genotoxicity of Hydrogen Peroxide and Antioxidant Action of Halo Acid by the Method of Dna-Comet with the Use of the Model of Transplantable Cell Cultures

DOI: https://doi.org/10.15688/jvolsu11.2018.1.7

 
Olga V. Verle
Postgraduate Student, Department of Theoretical Biochemistry with Profile in Clinical Biochemistry, Volgograd State Medical University
Pavshikh Bortsov Sq., 1, 400131 Volgograd, Russian Federation
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Oleg V. Ostrovskiy
Doctor of Sciences (Medicine), Professor, Head of Department of Theoretical Biochemistry with Profile in Clinical Biochemistry, Volgograd State Medical University
Pavshikh Bortsov Sq., 1, 400131 Volgograd, Russian Federation
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Valerian E. Verovskiy
Candidate of Sciences (Chemistry), Associate Professor, Department of Theoretical Biochemistry with Profile in Clinical Biochemistry, Volgograd State Medical University
Pavshikh Bortsov Sq., 1, 400131 Volgograd, Russian Federation
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Galina P. Dudchenko
Doctor of Sciences (Biology), Professor, Department of Theoretical Biochemistry with Profile in Clinical Biochemistry, Volgograd State Medical University
Pavshikh Bortsov Sq., 1, 400131 Volgograd, Russian Federation
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Abstract. In the framework of the study, the degree of defragmentation of DNA by the DNA-comet method 
is evaluated when exposed to the cell culture of hydrogen peroxide (H2O2), and an in vitro model is developed to 
evaluate the antioxidant activity of new pharmacological agents. The results of working with cell lines show that 
the percentage of damage to the genetic material of cells of intact samples does not greatly vary from the method 
of removing the cellular monolayer from the culture plastic. Concerning the effect of H2O2 as an inducer of 
oxidative stress on DNA cell damage, the optimal level of DNA defragmentation has been modeled for subsequent 
studies of the protective action of antioxidants.
 
Key words: antioxidants, oxidative stress, genotoxicity, hydrogen peroxide, antioxidant effect, gallic acid, DNA-comet method, model of transplantable cell cultures.
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Estimation of the Genotoxicity of Hydrogen Peroxide and Antioxidant Action of Halo Acid by the Method of Dna-Comet with the Use of the Model of Transplantable Cell Cultures by Verle O.V., Ostrovskiy O.V., Verovskiy V.E., Dudchenko G.P. is licensed under a Creative Commons Attribution 4.0 International License.
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